Non radioactive PRPP-S assay

PRECICE® PRPP-S Assay kit provides the first non radioactive and one-step protocol for measurement of PRPP-S activity in cellular lysates in a convenient 96-well plate format.

Phosphoribosylpyrophosphate synthetase (PRS; EC, an essential enzyme for the purine salvage pathway, is encoded by PRPS1 gene. Several mutations in this gene associated with genetic disorders have been described leading to PRS superactivity . This condition is inherited in an X-linked pattern.
PRPS1 gene overactivity increases the production of normal PRPP synthetase 1 enzyme, which increases the availability of PRPP. Excessive amounts of purines are generated leading to an accumulation of uric acid, a waste product of purine breakdown, in the body. A buildup of uric acid can cause gout, which is a form of arthritis resulting from uric acid crystals in the joints. Affected individuals may also develop kidney or bladder stones formed from uric acid crystals.
Increase of the availability of PRPP can be due to PRPP-S superactivity or HPRT deficiency (know more about HPRT assay ).


Ref. #K0709-04-2
#K0709-01-2 12 analyses (4 samples in triplicate) € 250.00 Inquiry

Pricing updated July 16th, 2014.

Kit is provided in stable lyophilized form and shipped without dry ice

Download Download PRPP-S assay Protocol (User manual)


Specific activitiy of PRS in lysates of human erythrocytes measured by PRECICE® PRPP-S Assay kit is close to previously published data.

PRPP-S assay graph
RBC speciment 1 (n=4) 65+/-2 nmol/hour/mg Hb
RBC speciment 2 (n=4) 76+/-12 nmol/hour/mg Hb
RBC speciment 3 (n=4) 78+/-10 nmol/hour/mg Hb
Published data R. B. Gordon et al J. Inher. Metab. Dis. 10 (1987) 82, 88 102+/-20nmol/hour/mg Hb


  • Non radioactive
  • Continuous
  • No sample preparation required. Cell lysates are directly used for continuous monitoring of PRPP-Synthetase activity


Simultaneous analysis of up to 6 samples in duplicate in 1h.

PRS activity is measured as a rate of production of PRPP with concomitant formation of NADH2 after two enzymatic reactions using highly active HPRT and IMPDH. The formation of NADH2 is continuously monitored spectrophotometrically at 340nm.