Synonyms: Hypoxanthine phosphoribosyltransferase, HGPRTase, HPRT
NOVOCIB'S human HGPRT enzyme is a 25kDa purified protein cloned by RT-PCR amplification of mRNA extracted from human hepatoma cells and expressed in E.coli. The sequence of cloned HGPRT (accession number P00492) was confirmed by DNA sequencing (100% identity).
NOVOCIB's cloned human HGPRT is a highly active enzyme that catalyzes the reversible transfer of the 5-phosphoribosyl group between α-D-5-phosphoribosyl-1-pyrophosphate (PRPP) and a purine base (hypoxanthine or guanine) to form a purine nucleotide IMP or GMP. The enzyme has been characterized for its affinity for natural substrates and velocity in forward and reverse directions.
Supplied in stable form (lyophilized), this enzyme provides an excellent positive control for in vitro HGPRT assay protocols when testing protein expression and activity in cell lysates.
| #REF | SIZE | PRICE | |
|---|---|---|---|
| #E-Nov9 | 100 mUnits - 4 tubes with 25 mUnits each (Once dissolved, 10 mU/ml correspond to 126 nmol/h/ml) |
€ 310.00 | Inquiry |
Pricing updated December 8th, 2013.
Kit is provided in stable lyophilized form and shipped without dry ice
You can ask us for a quotation here or write at contact@novocib.com
Storage: HPRT enzyme is provided in lyophilized form and should be stored at -20°C.
Unit Definition: (forward reaction) One unit of hypoxanthine-guanine phosphoribosyl transferase converts 1 µmole of hypoxanthine to IMP per minute at pH 8.8 at 25°C, as measured by a coupled IMPDH enzyme system. (see HPRT Assay Kit for further details)
Specific Activity: ≥ 1 Unit/mg protein.
Purity: controlled by 12%AA SDS-PAGE.
