PRECICE® AMP Deaminase Assay Kit
Non-radioactive, continuous spectrophotometric assay for precise measurement of AMP deaminase activity in biological samples
The PRECICE® AMP Deaminase Assay Kit offers researchers a non-radioactive, one-step protocol for accurate measurement of AMP deaminase (AMPD) activity in cellular lysates. This innovative kit utilizes a continuous spectrophotometric method in a convenient 96-well plate format, eliminating the need for hazardous radioactive materials while providing real-time kinetic data.
Key Benefits:
- Non-radioactive - Safe and easy to use without special handling requirements
- Continuous monitoring - Real-time kinetic measurement of enzyme activity
- High sensitivity - Detects low levels of AMPD activity in various sample types
- No sample preparation - Direct analysis of cell lysates without purification steps
PRECICE® AMP Deaminase Assay Kit
#K0709-05-2 | 96 tests/kit
PRECICE® AMP Deaminase Assay Kit
- #K0709-05-2 - 96 tests/kit
- Includes active AMP deaminase control
- Shipped at ambient temperature
- 12 months shelf life at -20°C
Kit provided in stable lyophilized form and shipped without dry ice
Validation Data
Performance Characteristics
| Parameter | Value |
|---|---|
| Sample Type | Human erythrocyte lysates |
| Specific Activity (RBC specimen 1) | 216 ± 1 nmol/hour/mg Hb (n=2) |
| Specific Activity (RBC specimen 2) | 226 ± 5 nmol/hour/mg Hb (n=2) |
| Linearity | Up to 200 mU/mL (R² > 0.99) |
| Sensitivity | Detects as low as 0.5 mU/mL |
Key Features & Benefits
Non-radioactive
Safe and easy-to-use assay without the need for special handling or disposal of radioactive materials.
Continuous Monitoring
Real-time kinetic measurement of enzyme activity with continuous monitoring of NADH formation at 340 nm.
Minimal Sample Preparation
Direct analysis of cell lysates without the need for purification or concentration steps.
Assay Principle
AMP Deaminase (AMPD) catalyzes the hydrolytic deamination of adenosine monophosphate (AMP) to inosine monophosphate (IMP) and ammonia. The PRECICE® AMP Deaminase Assay Kit measures this activity through a coupled enzymatic reaction:
- 1. AMP Deaminase Reaction:
AMP + H₂O → IMP + NH₄⁺ - 2. IMP Dehydrogenase Reaction:
IMP + NAD⁺ → XMP + NADH + H⁺
The formation of NADH is continuously monitored by the increase in absorbance at 340 nm, providing a direct and quantitative measure of AMP deaminase activity.
Please, Contact us for any questions.
Or ask us by email: contact@novocib.com
Applications in Research
Exercise Physiology
Study the role of AMP deaminase in energy metabolism during physical activity and muscle fatigue.
Metabolic Disorders
Investigate AMPD1 deficiency and its association with metabolic myopathies and exercise intolerance.
Drug Discovery
Screen for modulators of AMP deaminase activity as potential therapeutic agents.
Scientific Research & Publications
The PRECICE® AMP Deaminase Assay Kit has been utilized in numerous peer-reviewed studies, demonstrating its reliability and effectiveness in AMP deaminase research:
Muscle AMP deaminase activity was lower in Neandertals than in modern humans
2025Nature Communications 16(1) - Macak, D., Lee, S.Y., Nyman, T., et al.
DOI: 10.1038/s41467-025-61605-4Discovery of N-Arylsulfonyl-Indole-2-Carboxamide Derivatives as Fructose-1,6-Bisphosphatase Inhibitors
2020Journal of Medicinal Chemistry 63(18) - Zhou, J., Bie, J., Wang, X., et al.
DOI: 10.1021/acs.jmedchem.0c00726Need More Information?
Our technical support team is ready to assist you with any questions about the PRECICE® AMP Deaminase Assay Kit.