Non radioactive AMP Deaminase Assay

Including active AMP-DA

PRECICE® AMP Deaminase Assay kit provides the first non radioactive and one-step protocol for measurement of AMP Deaminase activity in cellular lysates in a convenient 96-well plate format.

PRECICE® AMP Deaminase Assay Kit

#K0709-05-2
#REF SIZE PRICE
#K0709-05-2 12 analyses (6 ml reaction buffer) € 210.00 Inquiry
#K0709-05-2 24 analyses (12 ml reaction buffer) € 410.00 Inquiry

* Pricing updated March 3rd, 2022.

Kit provided in stable lyophilized form and shipped without dry ice

Download AMPD assay Protocol (User manual)

Validated

Specific activitiy of AMP Deaminase in lysates of human erythrocytes measured by PRECICE® AMP Deaminase Assay kit.

AMPD Reaction Graph
RBC speciment 1 (n=2) 216+/-1 nmol/hour/mg Hb
RBC speciment 2 (n=2) 226+/-5 nmol/hour/mg Hb

Convenient

  • Non radioactive
  • Continuous
  • No sample preparation required. Cell lysates are directly used for continuous monitoring of AMP Deaminase activity

Fast

Simultaneous analysis of up to 12 samples in duplicate in 1h.

Principle
AMP Deaminase activity is measured as a rate of production of IMP with concomitant formation of NADH2 after one enzymatic reaction using highly active IMPDH. The formation of NADH2 is continuously monitored spectrophotometrically at 340nm.

Please, Contact us for any questions.

Or ask us by email: contact@novocib.com