Human deoxycytidine kinase enzyme has a broad substrate specificity and provides the phosphorylation of purine/pyrimidine deoxynucleosides and pyrimidine ribonucleosides. Deoxycytidine kinase is critical for phosphorylation of cytidine analogs and provides first steps of activation of highly effective anti-cancer prodrugs, such as aracytidine, gemcitabine and cladribine.
PRECICE® dCK Phosphorylation Assay Kit is a first tool for rapid evaluation of substrates properties of novel nucleoside analogues for human deoxycytidine kinase.
PRECICE® dCK Phosphorylation Assay Kit is based on competitive inhibition of deoxyinosine (dIR) phosphorylation by dCK enzyme. In the absence of nucleoside competitor, deoxycytidine kinase phosphorylates deoxyinosine resulting in the formation of dIMP. dIMP is further oxidized by IMPDH to dXMP and NADH2 continuously monitored at 340nm. In the presence of nucleoside competitor, the phosphorylation of deoxyinosine, poor dCK substrate, is inhibited detected as a decrease in NADH2 formation.
#REF | SIZE | PRICE | |
---|---|---|---|
#K0307-01 | 1 plate (96 assays) | 420.00 € | Inquiry |
Updated on October 3rd, 2024.
Kit is provided in stable lyophilized form and shipped without dry ice
Screening service by NOVOCIB also available
For HTS evaluation of dCK activity in lysates, NOVOCIB offers dCK Assay Kit
Download dCK Phosphorylation Assay protocol (User manual)
Such as gemcitabine, cladribine, aracytidine, efficiently phosphorylated by tumor cells and natural pyrimidine and purine nucleosides.
Nucleoside Analogues | Deoxycytidine | Cladribine | Aracytidine | Gemcitabine | Fludarabine | Deoxyguanosine |
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IC50 (Competitive Inhibition) |
5µM | 30µM | 30µM | 100µM | 400µM | 1,2mM |
Homogenous and continuous (add-and-measure)
The assay allows the test of 12 analogues at 7 different concentrations at the same time (or 6 analogues in duplicata)
Readout is peformed with plate reader fitted with 340nm filter