Continuous Phosphatase Assay
PRECICE® Phosphatase Assay Kit developed by NOVOCIB is a continuous spectrophotometric enzyme assay to monitor activity of any enzymes liberating inorganic phosphate (Pi) during enzymatic reactions. The kit can be used for:
- Phosphatase activity assay
- Alkaline phosphatase activity
- Protein phosphatases activity assay
- ATPase assay
- GTPases assay
- Helicases assay.
Principle:
The release of inorganic phosphate is measured through coupling to PNP-XDH enzymatic system. In the presence of inorganic phosphate, PNP (purine nucleoside phosphorylase) catalyzes inosine phosphorolysis leading to the formation of hypoxanthine and P-ribose. XDH enzyme catalyzes irreversible oxidation of hypoxanthine to uric acid with simultaneous reduction of NAD to NADH2, measurable by absorbance at 340nm. The formation of NADH2 is continuously monitored spectrophotometrically at 340nm. The assay is linear in 5-300µM range.
PRECICE® Phosphatase Assay Kit
Ref. #K0211-01
| #REF | SIZE | PRICE | |
|---|---|---|---|
| #K0211-01 |
PRECICE® Phosphatase Assay Kit 1 plate (96 assays) |
410.00 € |
410.00 €
Inquiry
|
Pricing updated November 25th, 2024.
Kit is provided in stable lyophilized form and shipped without dry ice
You can ask us for a quotation Here or write at contact@novocib.com
Download Our User Manual
🔬 Key Features of the Assay Kit
☑️ Convenient
- Non-radioactive
- Homogenous
- Continuous absorbance monitoring at 340 nm
- Tolerant to inorganic phosphate contamination
- Wide pH range
- Compatible with reducing agents
📊 Quantitative
- IMP transformed into NADH₂ for kinetic analysis
- Wide linearity range (5–300 µM)
🔁 Continuous
- Activity tracked via absorbance at 340 nm
- Supports real-time monitoring
⚙️ High-Throughput Analysis
- Performed in standard multi-well microplates
- Readout with plate reader fitted with 340 nm filter
🎯 Specific
- Quantifies only inorganic phosphate