Continuous Phosphatase Assay
PRECICE® Phosphatase Assay Kit developed by NOVOCIB is a continuous spectrophotometric enzyme assay to monitor activity of any enzymes liberating inorganic phosphate (Pi) during enzymatic reactions. The kit can be used for:
- Phosphatase activity assay
- Alkaline phosphatase activity
- Protein phosphatases activity assay
- ATPase assay
- GTPases assay
- Helicases assay.
Principle:
The release of inorganic phosphate is measured through coupling to PNP-XDH enzymatic system. In the presence of inorganic phosphate, PNP (purine nucleoside phosphorylase) catalyzes inosine phosphorolysis leading to the formation of hypoxanthine and P-ribose. XDH enzyme catalyzes irreversible oxidation of hypoxanthine to uric acid with simultaneous reduction of NAD to NADH2, measurable by absorbance at 340nm. The formation of NADH2 is continuously monitored spectrophotometrically at 340nm. The assay is linear in 5-300µM range.
PRECICE® Phosphatase Assay Kit
Ref. #K0211-01
| #REF | SIZE | PRICE | |
|---|---|---|---|
| #K0211-01 | 1 plate (96 assays) | € 410.00 | Inquiry |
Pricing updated December 25th, 2013.
Kit is provided in stable lyophilized form and shipped without dry ice
You can ask us for a quotation Here or write at contact@novocib.com
Download Our User Manual
Key Features
Convenient
- Non-radioactive
- Homogenous
- Continuous absorbance monitoring at 340nm
Quantitative
- The IMP is immediately transformed into NADH2 in a quantitative manner allowing for accurate kinetic analysis
Continuous
- Activity is measured through continuous montoring of absorbance at 340nm
Convenient
- Tolerant to contamination of ware and reagents by inorganic phosphate
- Homogenous
- Non radioactive
- Wide pH range
- Compatible with reducing agents
Quantitative
- Wide linearity range (5-300µM)
High-Throughput Analysis
- The assay is performed in standard multi-well microplates
- Non Readout is peformed with plate reader fitted with 340nm filter
Specific
- Only inorganic phosphate is quantified