• 
NOVOCIB proposes two Nucleoside Kinase Assay Kits to directly measure, through a simple spectrophotometric Assay, Adenosine Kinase activity or Deoxycytidine Kinase activity, in vitro. These Nucleoside Kinase Assay Kits are based on a couped reaction with a highly active IMPDH (Ref.#E-Nov7). |
PRECICE® ADK Screening Assay Kit
NOVOCIB's ADK Screening Assay Kit is designed to measure the activity of AK (adenosine kinase) in vitro.
This very easy one-step Assay is based on the use of inosine (IR) as a substrate of AK and a coupled reaction involving a highly active IMPDH (Inosine Monophosphate Dehydrogenase, bacterial recombinant, Ref.#E-Nov7) for a direct measurement of the inosine monophosphate (IMP) formed by AK.
A first reaction consists in the phosphorylation of IR by AK, in the presence of ATP, leading to the formation of IMP and ADP. IMP is then oxidized to xanthosine monophosphate (XMP) by IMPDH. This second reaction is immediate when IMPDH activity is much higher than AK activity in the assay.
The enzymatic activity of AK, which corresponds to the formation kinetics of dIMP, is then stoichiometrically and directly monitored by the formation kinetics of NADH2.
This very easy one-step Assay is based on the use of inosine (IR) as a substrate of AK and a coupled reaction involving a highly active IMPDH (Inosine Monophosphate Dehydrogenase, bacterial recombinant, Ref.#E-Nov7) for a direct measurement of the inosine monophosphate (IMP) formed by AK.
A first reaction consists in the phosphorylation of IR by AK, in the presence of ATP, leading to the formation of IMP and ADP. IMP is then oxidized to xanthosine monophosphate (XMP) by IMPDH. This second reaction is immediate when IMPDH activity is much higher than AK activity in the assay.
The enzymatic activity of AK, which corresponds to the formation kinetics of dIMP, is then stoichiometrically and directly monitored by the formation kinetics of NADH2.
For AK inhibitor screening services, see also our
AK Inhibition Assay Service
AK Inhibition Assay Service
NOVOCIB's dCK Assay Kit is designed to measure the activity of dCK (deoxycytidine kinase) in vitro.
This very easy one-step Assay is based on the use of deoxyinosine (dIR) as a substrate of dCK and a coupled reaction involving a highly active IMPDH (Inosine Monophosphate Dehydrogenase, bacterial recombinant, Ref.#E-Nov7) for a direct measurement of the deoxyinosine monophosphate (dIMP) formed by dCK.
A first reaction consists in the phosphorylation of dIR by dCK, in the presence of ATP, leading to the formation of dIMP and ADP. dIMP is then oxidized to deoxyxanthosine monophosphate (dXMP) by IMPDH. This second reaction is immediate when IMPDH activity is much higher than dCK activity in the assay.
The enzymatic activity of dCK, which corresponds to the formation kinetics of dIMP, is then stoichiometrically and directly monitored by the formation kinetics of NADH2.
This very easy one-step Assay is based on the use of deoxyinosine (dIR) as a substrate of dCK and a coupled reaction involving a highly active IMPDH (Inosine Monophosphate Dehydrogenase, bacterial recombinant, Ref.#E-Nov7) for a direct measurement of the deoxyinosine monophosphate (dIMP) formed by dCK.
A first reaction consists in the phosphorylation of dIR by dCK, in the presence of ATP, leading to the formation of dIMP and ADP. dIMP is then oxidized to deoxyxanthosine monophosphate (dXMP) by IMPDH. This second reaction is immediate when IMPDH activity is much higher than dCK activity in the assay.
The enzymatic activity of dCK, which corresponds to the formation kinetics of dIMP, is then stoichiometrically and directly monitored by the formation kinetics of NADH2.
For dCK inhibitor screening services, see also our
dCK Inhibition Assay Service
(^Top)
dCK Inhibition Assay Service