Synonyms: ADK, Adenosine 5'-phosphotransferase
NOVOCIB's human adenosine kinase is an active and purified, 345-aa short form(14,2) ADK protein (39kDa) cloned by RT-PCR amplification of mRNA extracted from human hepatoma cells and expressed in E.coli. The sequence of the cloned ADK (GenBank accession number U50196) was confirmed by DNA sequencing (100% identity)
Adenosine kinase is a key enzyme of nucleoside analogs activation
For rapid evaluation of substrates properties of novel nucleoside analogs for human adenosine kinase see our PRECICE® ADK Phosphorylation Assay Kit.
ADK is also a validated target for Drug Discovery: adenosine-regulating drugs have been extensively tested(1) as new analgesic and anti-inflammatory agents(4) to treat schizophrenia(7) or to limit brain injury after an ischemic stroke(8).
#REF | SIZE | PRICE | |
---|---|---|---|
#E-Nov5-100 | 100 mUnits | € 355.00 | Inquiry |
#E-Nov5-200 | 200 mUnits | € 565.00 | Inquiry |
#E-Nov5-500 | 500 mUnits | € 1420.00 | Inquiry |
Pricing updated December 8th, 2013.
Kit is provided in stable lyophilized form and shipped without dry ice
You can ask us for a quotation Here or write at contact@novocib.com
Adenosine kinase is a ubiquitous enzyme that catalyzes the transfer of γ-phosphate from ATP to 5'-hydroxyl of adenosine (AR), an important modulator of central nervous system functions and a signal molecule involved in hypoxia, inflammation, and nociception. Together with adenosine deaminase, adenosine kinase regulates intra- and extracellular cellular adenosine concentration. Inhibition of adenosine kinase results in a selective increase of local adenosine concentrations and reduced seizure susceptibility and nociception in vivo(3). ADK dysfunction is involved in several pathologies, including diabetes(13), epilepsy(6), and cancer. Consequently, ADK emerges as a rational therapeutic target for Drug Discovery, and adenosine-regulating drugs have been extensively tested (1) as new analgesic and anti-inflammatory agents(4) to treat schizophrenia(7) or to limit brain injury after an ischemic stroke(8).
The X-ray crystallographic structure of human ADK has been described(9) and provides structural basis for rational design and optimisation of new ADK inhibitors. In addition, this enzyme is responsible for the phosphorylation and consequent clinical activity of several therapeutically useful nucleosides, including the antiviral drug ribavirin(10), immunosuppressive drug mizoribine(11), and anticancer C-nucleoside, tiazofurin(12).
Unit Definition: One unit of adenosine kinase converts 1.0 µmole of inosine and ATP to IMP and ADP per minute at pH 8 at 37°C, as measured by a coupled IMPDH enzyme system.
(see ADK Assay Kit for further details)
Specific Activity: ≥ 0.200 unit/mg protein.
Purity: controlled by 12% AA SDS-PAGE.
Assay condition: Enzymatic activity of adenosine kinase with particular nucleoside substrate is measured by spectrophotometric assays in a coupled lactate dehydrogenase / pyruvate kinase system. Assays were carried out at 37°C, at 50mM Tris-HCl pH7,6; 50mM KCl, 5mM MgCl2, 2.5mM ATP, 0.1mM NADH, 1mM phosphoenolpyruvate, 1mM DTT, PK 5U/ml, LDH 5U/ml. Reaction was followed in an iEMS Reader MF (Labsystems) microtiter plate reader at 340nm. Nucleosides, nucleotides, LDH and PK were purchased from Sigma-Aldrich.