- Apolar free heterocyclic bases adenine, guanine, cytosine, and uracil (RNA) or thymine (DNA);
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Apolar free ribo- and deoxyribonucleosides
purines: adenosine/deoxyadenosine, guanosine/deoxyguanosine, inosine;
pyrimidines: cytidine/deoxycystidine, uridine, thymidine; - Negatively charged free ribo- and deoxyribonucleotides mono-, di- and triphosphates (AMP/dAMP, GMP/dGMP, IMP, CMP/dCMP, UMP, dTMP, ADP/dADP, GDP/dGDP, CDP/dCDP, UDP, ATP/dATP, GTP/dGTP, CTP/dCTP, UTP)
- Polymeric negatively charged nucleic acids RNA and DNA composed of ribo- and deoxynucleotides monophosphates, respectively.
Nucleotides are present in cells in different forms:
NOVOCIB offers a range of analytical services from characterisation of free 5' nucleotides to full spectrum of nucleotides (RNA, DNA, NMP, nucleosides and bases) in feed/ food products and ingredients using ion-pair high-performance liquid chromatography with diode array detection (Agilent 1200 HPLC-UV).