Award
Dr Larissa Balakireva, CEO & Founder of NovoCIB, was awarded with the Trophy of
"Femmes en Or 2011, Femme de l'Innovation"
in September 2011
.

PRPP-S Assay Kit Principle

PRECICE® PRPP-S Assay Kit

Ref. #K0709-04-2
Size Price*
12 analyses (4 samples in triplicate) € 250.00
* Pricing updated July 16th, 2014.
Kit provided in stable lyophilized form and shipped without dry ice.

To buy PRPP-S Assay Kit click here or ask for Quotation


Download PRPP-S assay Protocol  
(User Manual)

Non radioactive PRPP-S assay

PRECICE® PRPP-S Assay kit provides the first non radioactive and one-step protocol for measurement of PRPP-S activity in cellular lysates in a convenient 96-well plate format.

Phosphoribosylpyrophosphate synthetase (PRS; EC 2.7.6.1), an essential enzyme for the purine salvage pathway, is encoded by PRPS1 gene. Several mutations in this gene associated with genetic disorders have been described leading to PRS superactivity. This condition is inherited in an X-linked pattern.
PRPS1 gene overactivity increases the production of normal PRPP synthetase 1 enzyme, which increases the availability of PRPP. Excessive amounts of purines are generated leading to an accumulation of uric acid, a waste product of purine breakdown, in the body. A buildup of uric acid can cause gout, which is a form of arthritis resulting from uric acid crystals in the joints. Affected individuals may also develop kidney or bladder stones formed from uric acid crystals.
Increase of the availability of PRPP can be due to PRPP-S superactivity or HPRT deficiency (know more about HPRT assay).


Validated

    Specific activitiy of PRS in lysates of human erythrocytes measured by PRECICE® PRPP-S Assay kit is close to previously published data

    PRPP-S activity in red blood cell lysate  
    RBC speciment 1 (n=4)
    65+/-2 nmol/hour/mg Hb
    RBC speciment 2 (n=4) 76+/-12 nmol/hour/mg Hb
    RBC speciment 3 (n=4) 78+/-10 nmol/hour/mg Hb

    Published data
    R. B. Gordon et al
    J. Inher. Metab. Dis. 10 (1987) 82, 88

    102+/-20nmol/hour/mg Hb

Convenient

    Non radioactive
    Continuous
    No sample preparation required. Cell lysates are directly used for continuous monitoring of PRPP-Synthetase activity

Fast

    Simultaneous analysis of up to 6 samples in duplicate in 1 h

Principle
PRS activity is measured as a rate of production of PRPP with concomitant formation of NADH2 after two enzymatic reactions using highly active HPRT and IMPDH. The formation of NADH2 is continuously monitored spectrophotometrically at 340nm.

Please, contact us for any questions.



Related Links
PRPP Assay
Bacterial Recombinant IMPDH
Non-radioactive HPRT Assay
HGPRT enzyme