Anticancer nucleosides phosphorylationHuman deoxycytidine kinase enzyme has a broad substrate specificity and provides the phosphorylation of purine/pyrimidine deoxynucleosides and pyrimidine ribonucleosides. Deoxycytidine kinase is critical for phosphorylation of cytidine analogs and provides first steps of activation of highly effective anti-cancer prodrugs, such as aracytidine, gemcitabine and cladribine. PRECICE® dCK Phosphorylation Assay Kit is based on competitive inhibition of deoxyinosine (dIR) phosphorylation by dCK enzyme. In the absence of nucleoside competitor, deoxycytidine kinase phosphorylates deoxyinosine resulting in the formation of dIMP. dIMP is further oxidized by IMPDH to dXMP and NADH2 continuously monitored at 340nm. In the presence of nucleoside competitor, the phosphorylation of deoxyinosine, poor dCK substrate, is inhibited detected as a decrease in NADH2 formation. Validated with anticancer nucleoside analogues• Such as gemcitabine, cladribine, aracytidine, efficiently phosphorylated by tumor cells and natural pyrimidine and purine nucleosides 
Simple
High-Throughput Analysis
• Readout is peformed with plate reader fitted with 340nm filter |
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| Related Links | |
| • dCK enzyme
• ADK Assay Kit • ADK enzyme |
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