Award
Dr Larissa Balakireva, CEO & Founder of NovoCIB, was awarded with the Trophy of
"Femmes en Or 2011, Femme de l'Innovation"
in September 2011
.

dCK phosphorylation principle

PRECICE® dCK Phosphorylation Assay Kit

Ref. #K0307-01
Size Price*
1 plate (96 assays) € 420.00

* Pricing updated December 25th, 2013.
Kit provided in stable lyophilized form and shipped without dry ice
To buy dCK Assay Kit click here or ask for Quotation for more samples.
Screening service by NOVOCIB also available
For HTS evaluation of dCK activity in lysates, NOVOCIB offers dCK Assay Kit

Download dCK Phosphorylation Assay protocol  

Anticancer nucleosides phosphorylation

Human deoxycytidine kinase enzyme has a broad substrate specificity and provides the phosphorylation of purine/pyrimidine deoxynucleosides and pyrimidine ribonucleosides. Deoxycytidine kinase is critical for phosphorylation of cytidine analogs and provides first steps of activation of highly effective anti-cancer prodrugs, such as aracytidine, gemcitabine and cladribine.

PRECICE® dCK Phosphorylation Assay Kit is a first tool for rapid evaluation of substrates properties of novel nucleoside analogues for human deoxycytidine kinase.


Validated with anticancer nucleoside analogues

    Such as gemcitabine, cladribine, aracytidine, efficiently phosphorylated by tumor cells and natural pyrimidine and purine nucleosides

dCK phosphorylation validation

Nucleoside Analogues

Deoxycytidine

Cladribine

Aracytidine

Gemcitabine

Fludarabine

Deoxyguanosine

IC50
(Competitive Inhibition)

5µM

30µM

30µM

100µM

400µM

1,2mM

Simple

    Homogenous and continuous (add-and-measure)

High-Throughput Analysis

    The assay allows the test of 12 analogues at 7 different concentrations at the same time (or 6 analogues in duplicata)
    Readout is peformed with plate reader fitted with 340nm filter

Principle
PRECICE® dCK Phosphorylation Assay Kit is based on competitive inhibition of deoxyinosine (dIR) phosphorylation by dCK enzyme.
In the absence of nucleoside competitor, deoxycytidine kinase phosphorylates deoxyinosine resulting in the formation of dIMP. dIMP is further oxidized by IMPDH to dXMP and NADH2 continuously monitored at 340nm.
In the presence of nucleoside competitor, the phosphorylation of deoxyinosine, poor dCK substrate, is inhibited detected as a decrease in NADH2 formation.



Related Links
dCK enzyme
ADK Assay Kit
ADK enzyme
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