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Nucleotide Analysis
 Mechanism Of Action by Nucleotide Profiling |
Baby Milk Nucleotide Analysis |
| Customized Nucleotide Profiling Analysis |
(Meat &) Fish Freshness Analysis |
Mechanism of Action by Nucleotide Profiles
NOVOCIB has developed a range of Nucleotide Profiling Services based on an accurate quantification by HPLC of more than 31 cellular (deoxy-) ribonucleotides (mono-, di-, and triphosphate) and nucleotide co-factors in drug-treated cells.
These analytical services were validated with a wide range of recognized inhibitors of nucleotide biosynthesis: MPA, hydroxyurea, methotrexate, ribavirin, mizoribine, leflunomide, gemcitabine… These reference drugs do impact differently on nucleotide metabolism, each showing its own mode of action through a specific nucleotide profile, which is used to fulfil a reference collection of metabolic signatures.
Any new compound's mode of action is analyzed by establishing its nucleotide profile which is then compared with those obtained with reference drugs.
| Assays | Targeted Enzyme(s)
/ Targeted Biosynthetic Pathway |
Reference Drugs (examples) |
| IMPDH inhibition:
Whole Cell Assay |
Inosine Monophosphate Dehydrogenase
(IMPDH) |
Ribavirin
MPA (Mycophenolic Acid) |
| RNR inhibition
Whole Cell Assay |
RNR (Ribonucleotide Reductase) | HU (Hydroxyurea
Gemcitabine |
| Multi-targeted Antifolates:
Whole Cell Assay |
• de novo Purine biosynthesis enzymes:
GAR transformylase, AICAR transformylase • Thymidylate Synthase (TS) |
MTX
(Methotrexate) |
These analytical services were validated with a wide range of recognized inhibitors of nucleotide biosynthesis: MPA, hydroxyurea, methotrexate, ribavirin, mizoribine, leflunomide, gemcitabine… These reference drugs do impact differently on nucleotide metabolism, each showing its own mode of action through a specific nucleotide profile, which is used to fulfil a reference collection of metabolic signatures.
Any new compound's mode of action is analyzed by establishing its nucleotide profile which is then compared with those obtained with reference drugs.
Download our brochure "Mode of Action Studies by Nucleotide Profiling" 
Other nucleotide analyses are available. Please, see also our Customized Nucleotide Profiling Analysis
Baby Milk Nucleotide Analysis (^Top)
Aim: Determination of the content of nucleotides monophosphate in baby milk powder, particularly CMP, UMP, GMP, IMP and AMP
Nucleotides are naturally present in milk (i.e. 2-5% of the non-protein nitrogen in Human milk). However, after studies had suggested that nucleotides have positive effects in infant nutrition, manufacturers have got used to adding nucleotides in baby food, for several years now and particularly in milk powder. Unsurprisingly, in a context where food additives are more and more subjected to regulation, nucleotide additions in baby milk powders do or are about to enter under the control of strict rules.
Nucleotides additives in baby food are generally monophosphate nucleotides. NOVOCIB analyzes CMP, UMP, GMP, IMP and AMP contents in baby milk. The analysis of other nucleotides is also available upon request.
Do not hesitate to contact us.
Nucleotides are naturally present in milk (i.e. 2-5% of the non-protein nitrogen in Human milk). However, after studies had suggested that nucleotides have positive effects in infant nutrition, manufacturers have got used to adding nucleotides in baby food, for several years now and particularly in milk powder. Unsurprisingly, in a context where food additives are more and more subjected to regulation, nucleotide additions in baby milk powders do or are about to enter under the control of strict rules.
Nucleotides additives in baby food are generally monophosphate nucleotides. NOVOCIB analyzes CMP, UMP, GMP, IMP and AMP contents in baby milk. The analysis of other nucleotides is also available upon request.
Do not hesitate to contact us.
To know more about NovoCIB's services for Nucleotide Analysis in BabyMilk
(Meat &) Fish Freshness Analysis (^Top)
Aim: Determination of the level of freshness of foodstuffs, especially for fish and seafood.
The K value concept: The concept was first proposed by a Japanese research team in the late 1950's and has since been the subject of numerous studies. "K value" is now considered as the most effective and objective indicator of the freshness of fish, and to an extent other seafood and meat (beef, pork, lamb, poultry...).
"K value" is based on ATP breakdown due to respiratory cell death, and the subsequent formation of nucleotide by-products, namely inosine monophosphate (IMP), inosine (Ino) and, at a later stage, hypoxanthine (Hx). K-value measures how far ATP degradation has progressed within a tissue, thus, in other words, the level of freshness of the flesh.
In the value chain of fish and seafood industry, K-value is particularly relevant at the early stages of storage and processing, during autolysis and before spoilage starts.
NOVOCIB's expertise in nucleotide quantification led to the development of an analytical method specially designed for the "K value" measurement of fish sample.
The K value concept: The concept was first proposed by a Japanese research team in the late 1950's and has since been the subject of numerous studies. "K value" is now considered as the most effective and objective indicator of the freshness of fish, and to an extent other seafood and meat (beef, pork, lamb, poultry...).
"K value" is based on ATP breakdown due to respiratory cell death, and the subsequent formation of nucleotide by-products, namely inosine monophosphate (IMP), inosine (Ino) and, at a later stage, hypoxanthine (Hx). K-value measures how far ATP degradation has progressed within a tissue, thus, in other words, the level of freshness of the flesh.
In the value chain of fish and seafood industry, K-value is particularly relevant at the early stages of storage and processing, during autolysis and before spoilage starts.
NOVOCIB's expertise in nucleotide quantification led to the development of an analytical method specially designed for the "K value" measurement of fish sample.
To know more about (Meat &) Fish Freshness Analysis by NovoCIB.
Customized Nucleotide Profiling Analysis (^Top)
Aims: Analysis of the Nucleoside & Nucleotide content in biological samples. From a one-compound analysis (e.g. ADP, ATP, NAD, NADP…) to a whole spectrum of nucleosides and nucleotides.
A wide range of samples are routinely analyzed such as cultured cells, blood cells, body fluids... Other materials can be subjected to a feasibility study before being analyzed. Please feel free to contact us for any question.
A wide range of samples are routinely analyzed such as cultured cells, blood cells, body fluids... Other materials can be subjected to a feasibility study before being analyzed. Please feel free to contact us for any question.
To know more about NovoCIB's Customized Nucleotide Profiling Analysis.
| Related Links | |
| • Purine Metabolism Enzymes
• Purine Metabolism Enzyme Assays |
• Nucleoside Kinase Assays
• Nucleoside Kinases |