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Mechanism Of Action (MOA) by Nucleotide Profiling - Contract Research Services
These Contract Research Services are also reported in the section dedicated to "Inhibitor Screening" and refered as "Whole Cell Screening".
IMPDH inhibition: Whole Cell Screening
RNR inhibition: Whole Cell Screening
Multi-targeted antifolates: Whole Cell Screening
To study the effect of nucleoside analogues on the whole spectra of cellular purine and pyrimidine ribo- and deoxyribonucleotides, NOVOCIB has developed an original cell-based analytical approach in which more than 31 (deoxy)ribonucleotides (mono-, di-, triphosphate) and nucleotide co-factors are extracted from cultured cells, separated by ion-pared chromatography and quantified.
These cellular assays were validated with anti-viral and anti-cancer nucleoside analogues (e.g. ribavirin, gemcitabine) and known anti-metabolites (mycophenolic acid, leflunomide, hydroxyurea, methotrexate).
These Contract Research Services are also reported in the section dedicated to "Inhibitor Screening" and refered as "Whole Cell Screening".
IMPDH inhibition: Whole Cell Screening
RNR inhibition: Whole Cell Screening
Multi-targeted antifolates: Whole Cell Screening
To study the effect of nucleoside analogues on the whole spectra of cellular purine and pyrimidine ribo- and deoxyribonucleotides, NOVOCIB has developed an original cell-based analytical approach in which more than 31 (deoxy)ribonucleotides (mono-, di-, triphosphate) and nucleotide co-factors are extracted from cultured cells, separated by ion-pared chromatography and quantified.
These cellular assays were validated with anti-viral and anti-cancer nucleoside analogues (e.g. ribavirin, gemcitabine) and known anti-metabolites (mycophenolic acid, leflunomide, hydroxyurea, methotrexate).
Other nucleotide analyses are available. Please, see also our Customized Nucleotide Profiling Analysis
IMPDH inhibition: Whole Cell Screening
Aim: This Research Contract Service has been specially tailored to validate Inosine Monophosphate Dehydrogenase (IMPDH) inhibition by a given compound in cultured cells. The assay consists in extracting, identifying and quantifying by HPLC the intracellular concentration of guanosine nucleotides (GMP, GDP and GTP) and IMP in cells treated by a compound.
Whole Cell Screening for IMPDH Inhibition was validated with mycophenolic acid (MPA), ribavirin and mizoribin, recognized inhibitors of IMPDH. When applied for the study of nucleoside analogues (NA), this assay can also reveal the formation of their mono-, di-, and triphosphate forms, indicating that nucleoside analogues enter the cells and are readily phosphorylated by cellular kinases.
Whole Cell Screening for IMPDH Inhibition was validated with mycophenolic acid (MPA), ribavirin and mizoribin, recognized inhibitors of IMPDH. When applied for the study of nucleoside analogues (NA), this assay can also reveal the formation of their mono-, di-, and triphosphate forms, indicating that nucleoside analogues enter the cells and are readily phosphorylated by cellular kinases.
To know more about NovoCIB's IMPDH inhibition: Whole Cell Screening
RNR inhibition: Whole Cell Screening (^Top)
Aim: This Research Contract Service has been specially tailored to validate Ribonucleotide Reductase (RNR) inhibition by a given compound in cultured cells. The assay consists in extracting, identifying and quantifying by HPLC the intracellular concentration of deoxynucleotides di- and triphosphate in cells treated by a compound.
Whole Cell Screening for RNR Inhibition was validated with hydroxyurea and gemcitabine in HeLa cultured cells.
Whole Cell Screening for RNR Inhibition was validated with hydroxyurea and gemcitabine in HeLa cultured cells.
To know more about NovoCIB's RNR inhibition: Whole Cell Screening
Multi-targeted Antifolates: Whole Cell Screening (^Top)
Aim: This Research Contract Service has been specially tailored to validate inhibition of de novo biosynthesis of purine and pyrimidne nucleotides by a given compound in cultured cells. After incubation of cultured cells with the inhibitor, nucleotides are extracted, separated, identified and quantified by UV-HPLC.
Whole Cell Screening for Multi-targeted Antifolates was validated with methotrexate in HeLa cultured cells.
Whole Cell Screening for Multi-targeted Antifolates was validated with methotrexate in HeLa cultured cells.
To know more about NovoCIB's Multi-targeted antifolates: Whole Cell Screening