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Enzymes & Assay Kits
NOVOCIB produces recombinant nucleoside kinases and other nucleotide metabolism enzymes and develops original biochemical assays based on these enzymes. This portfolio of enzymes and assays represents a unique range of powerful tools for Drug Discovery and research in the field of nucleoside and nucleotide metabolism.
NOVOCIB produces recombinant nucleoside kinases and other nucleotide metabolism enzymes and develops original biochemical assays based on these enzymes. This portfolio of enzymes and assays represents a unique range of powerful tools for Drug Discovery and research in the field of nucleoside and nucleotide metabolism.
 Purine Metabolism Enzymes
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Bacterial Bioluminescence Enzymes
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HPRT Assay Kit PRPP Assay Kit
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| Nucleoside Kinases
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Nucleoside Kinases Assay Kits
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NADPH, NADH Bioluminescence Assay Kit
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| IMPDH | Inosine Monophosphate Dehydrogenase (E.C.1.1.1.205) | Human Type II, recombinant, expressed in E.coli
Bacterial (Staphylococcus aureus) recombinant, expressed in E.coli |
| PNP | Purine Nucleoside Phosphorylase (E.C.2.4.2.1) | Human recombinant, expressed in E.coli |
| HGPRT | Hypoxanthine-guanine phosphoribosyltransferase (E.C.2.4.2.8) | Human recombinant, expressed in E.coli |
To know more about NovoCIB's Purine Metabolism Enzymes
| AK enzyme | Adenosine Kinase (E.C.2.7.1.20) | Human, recombinant, expressed in E.coli |
| dCK enzyme | Deoxycytidine Kinase (E.C.2.7.1.74) | Human recombinant, expressed in E.coli |
| CMK enzyme | UMP-CMP Kinase (E.C.2.7.4.14) | Human recombinant, expressed in E.coli |
| cN-II enzyme | cytosolic 5'-nucleotidase II (E.C.3.1.3.5) | Human recombinant, expressed in E.coli |
To know more about NovoCIB's Nucleoside Kinases
NOVOCIB's HGPRT Assay Kit is designed to measure the activity of HGPRT (hypoxanthine-guanine phosphoribosyl transferase) in vitro. This very easy one-step Assay is based on a coupled reaction involving a highly active IMPDH (Inosine Monophosphate Dehydrogenase, bacterial recombinant) which directly measures by spectrophotometry, through the IMP formed by HGPRT, the activity of HGPRT present in a sample. HGPRT Assay Kit was validated with Red Blood Cell lysate.
To know more about NovoCIB's HPRT Activity
Nucleoside Kinase Assay Kits (^Top)
| ADK Screening Assay Kit | For a direct measurement of Adenosine Kinase Activity by spectrophotometry |
| dCK Assay Kit | For a direct measurement of Deoxycytidinae Kinase Activity by spectrophotometry |
To know more about NovoCIB's Nucleoside Kinase Assay Kits
NOVOCIB's PRPP Assay Kit is designed to measure PRPP (α-D-5-phosphoribosyl-1-pyrophosphate) content in samples. This enzymatic assay is based on a coupled reaction involving Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and Inosine Monophosphate Dehydrogenase (IMPDH).
PRPP is highly unstable, which renders the preparation of accurately weighted standards difficult. This PRPP Assay Kit has the advantage of measuring PRPP concentration in the sample by absorbance through a stoichiometric NADH formation, thus allowing a direct and absolute measurement of PRPP content with no need of any calibration curve.
To know more about NovoCIB's HPRT Activity
Bacterial Bioluminescence Enzymes (^Top)
Bacterial bioluminescence consists in a ligh-emitting reaction where a long-chain aliphatic aldehyde is oxidized in the presence of molecular oxygen and reduced flavin mononucleotide (FMNH2). This reaction is catalyzed by bacterial luciferase.
NOVOCIB offers a highly pure bacterial luciferase from Photobacterium phosphoreum and a recombinant FMN-Reductase which, when coupled to bacterial luciferase in vitro, is able to significantly increase the sensitivity and allow a better control of signal intensity and duration.
NOVOCIB offers a highly pure bacterial luciferase from Photobacterium phosphoreum and a recombinant FMN-Reductase which, when coupled to bacterial luciferase in vitro, is able to significantly increase the sensitivity and allow a better control of signal intensity and duration.
| Bacterial Luciferase | (E.C.1.14.14.3) | Bacterial, produced from a selected strain of Photobacterium phosphoreum |
| FMN Reductase | (E.C.1.5.1.29) | Bacterial recombinant, E.coli |
To know more about NovoCIB's Bacterial Bioluminescence Enzymes
NADPH, NADH Bioluminescence Assay Kit (^Top)
NOVOCIB’s NADPH, NADH Bioluminescent Kit provides an ultrasensitive, one-step assay to detect as low as 2.10-13 mole of NAD(P)H in a 100µL assay volume (2nM). This kit is based on two major enzymes involved in bacterial luminescence mechanism: bacterial luciferase and a recombinant FMN reductase.
To know more about NovoCIB's NADPH, NADH Bioluminescence Assay Kit