Award
Dr Larissa Balakireva, CEO & Founder of NovoCIB, was awarded with the Trophy of
"Femmes en Or 2011, Femme de l'Innovation"
in September 2011
.


Bacterial IMPDH
Ref. #E-Nov7

Quantity Price*
50 mUnits € 195.00

* Pricing updated December 12th, 2013

Provided in stable lyophilized form and shipped without dry ice
To buy bacterial IMPDH enzyme click here or ask for Quotation

Bacterial IMPDH (Staphylococcus aureus)
(EC 1.1.1.205)

Synonyms: inosine 5'-monophosphate dehydrogenase, IMP dehydrogenase

NOVOCIB's bacterial IMPDH is a cloned protein of 53kDa cloned by PCR amplification of guaB gene of Staphylococcus aureus and expressed in E. coli.

Today, antibiotic resistance is one of the world’s most important public health problems. There is an urgent need for new antibiotic compounds acting on new targets. One attractive strategy for developing new antibiotics consists in inhibiting bacterial IMPDH, an enzyme involved in the de novo synthesis of purine nucleotides, and therefore, necessary for bacterial cell growth and division.

Mammalian and bacterial IMPDHs are known to have significantly different kinetic properties and inhibitor sensitivities(1,2). The experiments done with previously cloned human IMPDH2 and bacterial IMPDH of Staphylococcus aureus, are illustrated below. In agreement with published data, mycophenolic acid (MPA) inhibits human IMPDH type II >20-times more efficiently than bacterial IMPDH with IC50 values of 100nM and 2.6µM respectively (A). In contrast, mizoribine monophosphate displays the opposite selectivity (B). It is a more potent inhibitor of bacterial IMPDH with respective IC50 values of 12nM and 185nM for bacterial and human enzymes.




Bacterial recombinant IMPDH and human recombinant IMPDH, Type 2, enzymes are useful tools for the selection of species-specific IMPDH inhibitors. Both enzymes are available at NOVOCIB.

IMPDH inhibition: Effect of MPA (A) and mizoribine monophosphate (B) on human recombinant IMPDH II (red curve) and bacterial recombinant IMPDH of Staphylococcus aureus (blue curve). Enzymatic assays are performed in duplicate at 37°C in 0.1M KH2PO4 buffer pH 8.0 in the presence of 1mM DTT, 200µM NAD, 200µM IMP, 60nM Human IMPDH, Type 2 or 95nM IMPDH S.aureus. Reaction is followed in an iEMS Reader MF (Labsystems) microtiter plate reader at 340nm.

Monophosphorylated mizoribine is produced enzymatically by phosphorylation of mizoribine (MP Biochemicals) using NOVOCIB's adenosine kinase (AK).
Unit Definition: One unit of IMPDH converts 1.0 µmole of IMP and NAD to XMP and NADH per minute at pH 8 at 37°C. Specific Activity: ≥ 0.3 unit/mg protein. Purity: controlled by 12%AA SDS-PAGE.
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References (with links to PubMed)
1. L. Hedstrom and L. Gan (2006): IMP dehydrogenase: structural schizophrenia and an unusual base Curr. Opin. Chem. Biol. 10(5), 520-525
2. R. Zhang et al. (1999): Characteristics and crystal structure of bacterial inosine-5'-monophosphate dehydrogenase Biochemistry 13;38(15), 4691-700



Related Links
Purine Metabolism Enzymes
Human Recombinant IMPDH
Nucleoside Kinase Assay Kits
HPRT Assay
PRPP Assay
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